Udita Agrawal has completed her M. Pharm. at the age of 26 years from Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar. Presently she is pursuing her Ph. D. in the supervision of Prof. S. P. Vyas at Dr. H. S. Gour Central University, Sagar, India. Ms. Udita Agrawal is a Gold medalist holder in B. Pharm and M. Pharm level. She has won various awards for the best oral and poster presentations. She has published 6 papers to her credit published in journals of high scientific impact.
Glioblastoma multiform (GBM), is characterized by high proliferation rate, migration, invasion as well as secretion of angiogenetic factors. Effective chemotherapy for glioblastoma requires a smart nanocarrier that can penetrate the blood brain barrier (BBB) and subsequently target the glioma cells. In the study dual-targeting paclitaxel (Ptx) polymeric nanoparticles (NPs) were produced by conjugating with both folate (FA) and cRGDfK, for the effective penetrating across BBB and targeting glioma, respectively. FA and cRGDfK modified NPs containing paclitaxel was prepared by nanoprecipitation technique combined with self-assembly. The PLNs was characterized by particle size, shape, zeta potential, entrapment efficiency, in vitro release profile, cytotoxicity, hemolysis study, cell uptake, in vitro transendothelial transport in brain endothelial cell lines. The in vivo anti- In vitro and in vivo studies demonstrated that the dual-targeting NPs could transport across the BBB and mainly distributed in the brain glioma. FA and cRGDfk conjugated NPs had maximum entrapment efficiency 81.34±3.41% (n=3). AFM showed NPs to be spherical and nanometric in size. The in vitro release followed in a biphasic pattern: i.e. an initial burst release followed by slower and sustained release and resulted in least haemolytic toxicity. The anti-tumor effect of the dual-targeting NPs also demonstrated increased survival time, decreased tumor volume and no significant effect on body weight. The dual-targeted NPs could improve the therapeutic efficacy of brain glioma and were found less toxic than the Ptx solution, showing a dual-targeting effect. These results indicate that dual-targeting NPs are promising potential carrier for glioma chemotherapy.
DorotaWatrobska-Swietlikowska has completed his Ph.D. at the age of 28 years from Medical University of Gdansk. She is the scientific at Department of Pharmaceutical Technology of Medical University. She has published 4 papers wherein two of them in reputed journals. She collaborate with hospitals for studies the physical stability of parenteral admixtures with high electrolytes concentrations.
Many active substances used as drugs are poorly water-soluble. In order to administer these drugs in the form of solution (such a form is required e.g. for intravenous application) usually to the formulation co-solvents or artificial surfactants are added. These excipients can be substituted by natural and biodegradable lipid substances – phospholipids and oils, which are used for years parenterally. The aim of the work was to characterize LE and WLD as potential parenteral carriers used for solubilization of two model drugs: carbamazepine and hydrocortisone. Both formulations (LE and WLD) contained glycerol (2.5%) and phospholipids (5% or 10% in WDL or 1.2%, 2.4% and 5% in LE), while LE contained also soya-bean oil (10%). Submicron dispersion of the oil (LE) and lecithin particles (WLD) were obtained by multi-stage homogenization process and the sterility of the formulations was acquired by autoclaving. In order to characterize the carriers different physicochemical properties were tested (pH and osmotic pressure of the formulation, Zeta-potential, size and polydispersity of the particles). To determine the solubility of drugs in the tested formulations, active substances were introduced into LE or WLD and mixed at room temperature. After 24h suspensions were centrifuged, filtered and the drug concentration was analyzed by HPLC. Physicochemical tests indicated, that both LE and WLD fulfill the requirements of the carrier intended for parenteral application. It was also confirmed, that the solubility of carbamazepine and hydrocortisone in WDL or LEis higher comparing to water. Solubility of hydrocortisone increasedfrom 0.3 mg/ml (water) to 0.9 mg/ml(LE containing 1.2% of lecithin and 10% of the oil) and to 2 mg/ml (WLD containing 5% of egg-lecithin). Also the solubility of carbamazepine in WDL was better than in LE, even if both formulations contained the same lecithin concentration (5%). It was also noticed thatregardless of the type of carrier (LE or WLD) and of the type of surfactant (egg, soya-bean lecithin or pure phospholipids) the increase in phospholipid concentration caused the increase in tested drug’s solubility.